Symposium “Plants in Health and Culture”
Priscilla Chunda , Natali Rianika Mustafa and Robert Verpoorte
Pharmacognosy Division, Section of Metabolomics, Institute of Biology. Leiden University
Eliciting the salicylic acid biosynthetic pathway in Arabidopsis thaliana cell suspension cultures.
Salicylic acid (SA) is a well known phytohormone reported to have may functions in the plant. Most research interest however, lies in the ability of SA to act as an endogenous signal for the activation of defense mechanisms associated with the establishment of systemic acquired resistance (SAR) in the plant. Nevertheless the complete SA biosynthetic pathway is still to be established. A study to elucidate this pathway using labeled-precursor feeding experiments will be done to map this pathway hence the need to increase the level of SA in the plant cells. In an attempt to further increase the amounts of SA in Arabidopsis thaliana cell cultures and thereby enable labelling experiments of the SA biosynthetic pathway, elicitation was done with Pythium aphanidermatum, filter sterilised yeast, cellulase, pectinase and Pseudomonas syringae.
The increase of free SA and glycosidically bound SA (SAG) in the elicited cells in mg/g FW (with all elicitors ) was found to be very marginal in comparison to the control cells leading to the conclusion that this cell line was not suited for the radiolabelling experiments planned to elucidate the SA biosynthetic pathway. Cellulase proved to be the most efficient elicitor for this cell line.