Symposium “Plants in Health and Culture”
1E.C.Ogbuadike, 2W.T.Chang , 2H. Korthout, 1T. Raharjo, 1Y.H. Choi, 1H.K. Kim, 1R. Verpoorte.
1Division of Pharmacognosy, Gorlaeus Laboratories, Einsteinweg 55, 2300 RA Leiden, The Netherlands.
2Plant Medicines TNO Nutrition and Health Wassenaarseweg 64, 2333 Al Leiden, The Netherlands
Competitive binding activity of new metabolites from cell suspension cultures of Cannabis sativa L. to cannabinoid receptors
After cannabinoid precursor feeding, cell suspension cultures of Cannabis sativa, produced metabolites, which bind significantly to the cannabinoid receptors (CB1 & CB2). An effect known to be produced only by Δ9 tetrahydrocannabinol (Δ9THC), some of its analogues and endogenous cannabinoids (cannabinoid-like compounds in mammalian body). CB2 binding activity was found for CHCl3 fraction of Olivetol fed cells harvested on 1st day after feeding while both CB1 and CB2 binding activity was found on the 7th day harvest. Control cells and Pythium aphanidermatum elicitated cells show no significant binding activity, although CHCl3 fraction of Pythium aphanidermatum elicitated cells show new metabolite peaks in HPLC and 1H NMR. The new metabolites with binding activity stand out as possible models for developing new drugs with controlled psychoactive effect for treatment of: (i) brain nerve cells, spinal cord and peripherial tissue disorder as CB1 is located in these organs (ii) immune system disorder as CB2 is found in the immune system. Further research is therefore going on for the identification of these metabolites.