Symposium “Plants in Health and Culture”
Fransisca M. Poerba1, Wouter A. Duetz2, Arno Hazekamp1, Rob Verpoorte1
1-Division of Pharmacognosy, Section of Metabolomics, Institute of Biology,
2-Enzyscreen BV, Biopartner Centrum, Leiden, The Netherlands
Cannabinoids are metabolized in organisms to a number of oxygenated metabolites, and many of them were reported exhibit similar behavioral effects to the parent compounds and as a consequence many studies need to be done. Therefore, the availability of cannabinoid metabolites as research material and reference compound is very important. It is proposed that microbial transformation systems could closely mimic most of phase I transformation of drugs observed in mammals, therefore, several strains of microorganisms were screened for the capability to hydroxylate some cannabinoids. Screening was done using micro titer plate miniaturized growth of large number of microbial strain collection. Acinetobacter calcoaceticus, Gordona rubropertintus, and a gram positive microorganism were found to be capable to perform biotransformation of cannabinoids into more polar metabolites, including side chain hydroxylation of A9-tetrahydrocannabinol, 4'-hydroxy-A9-tetrahydrocannabinol.