Symposium “Plants in Health and Culture”
Isolation of three iso-cis-a acids from isomerised Hop (Humulus lupulus) extract
Since iso-α-acids plays an important role in beer, there is a demand on the individual iso-cis or trans-cohumulone, humulone, and adhumulone. Several efforts have been made but still not given a proper method that can be applied for large-scale production. Centrifugal Partition Chromatography (CPC) method has been widely used for preparative separation of many complex compounds. This study deals with a CPC developed for the separation of three compounds of Hop iso-α-acids (Humulus lupulus). The separation was accomplished by injecting 500 mg isomerised extract of cis-iso-α-acids mixture, into two phase CPC system using Toluene / Ethanol / H2O (5:2:13). 2% Trifluoroacetic Acids was used as retainer and 13 mM Ammonia (pH 11) as eluter. Collected fraction yielded 50 mg iso-cis-cohumulone, 125 mg iso-cis-humulone, and 28 mg iso-cis-adhumulone (over 90% pure as determined by HPLC).